SPI-Chem Paraformaldehyde CAS #30525-89-4
|1 to 9||$10.00|
|10 and up||$9.00|
|Formula Weight:||Not available|
|Melting Point:||120-170° C / 248-338° F|
|Specific Gravity (H2O = 1):||1.08|
|Flash Point:||70° C / 158° F|
|Solubility:||Solubility: Soluble in hot water, and many organic solvents. Insoluble in water, ether, and alcohol.|
|Density:||0.88 g/cm3 at 25° C (lit.)|
Paraformaldehyde has documented uses as a disinfectant, fungicide, fixation reagent and in the preparation of formaldehyde. In fluorescence studies, paraformaldehyde has been used as a formalin fixative to fix cells and tissues.
For Use in Electron Microscopy
This is a fast acting fixative used in a number of transmission electron microscopy protocols and is most often used in conjunction with glutaraldehyde and osmium tetroxide, often times called Karnovsky's fixative.
Reference: Kamovosky, J Cell Bio., 27, 137A (1965)
When using a formalin as a fixative in electron microscopy, the formalin must not contain methanol. Most commercial formalin contains methanol to prevent the formaldehyde from polymerizing. In electron microscopy studies, a fresh formalin solution can be prepared as follows:
- Add desired amount of paraformaldehyde to a basic buffer or water.
- Heat while stirring to 55-60°C until it dissolves and forms the formaldehyde solution.
SPI Supplies offers complete ready-to-mix and use Karnovsky's Fixative Kits. We believe that many users of this fixative would find it far more convenient to purchase the needed chemicals in this easy to use economical kit than to worry about trying to maintain in fresh supply the individual components.