BioBond Adhesive 20ml,
Tissue Section AdhesiveProtocols for use:A 2% solution is used for the coating of slides.
For light microscopy and electron microscopy, the choice of specimen preparation is critical for the preservation of antigens in the sample. Of greatest importance in the preparation schedules are the specimen fixation and embedding. The protocol must satisfy the requirements for preservation of structural integrity and antigenicity.
Having prepared the tissue specimen for immunolabeling, it is then imperative to perform the incubations with a protocol designed to maximise the specific signal and minimise the background. Some incubation conditions may cause tissue sections to be removed from the glass slide. Typical tissue section adhesives such as poly-L-lysine, Elmer's glue, chrome alum, etc are not suitable for use with immunogold labeling because of the increased background caused by attraction of gold particles to the adhesive on the slide.
In addition, the surface of glass slides is uneven and is activated by the silicon tetrahedral structure. This provides active sites for absorption of proteins or reactions with chemicals and reagents. It is therefore important to minimise this possibility by coating the surface with a material that is of low reactivity towards reagents.
SPI Supplies now offers a special slide coating solution, BioBond™, which produces a very strong adhesion between the glass and the tissue section for subsequent incubations. BioBond coats the slide with a protective layer to minimise interaction of charged glass surface with reagents. This is also of particular importance for reproducibility of results because of the variations that occur between glass slides obtained from different sources and in different countries. It is particularly effective for use with severe incubating conditions such as those used in In-situ hybridization. BioBond is suitable for all kinds of tissue specimens including paraffin wax or resin sections, cell smears, cytospins or cryostat sections.