Gold Conjugate, Rabbit anti-Goat IgG, 5nm, 1ml
While most immuno gold work, with electron microscopy has been done in conjunction with TEM, there have been published some very exciting results based on the use of SEM as well. For example, there is great interest in using colloidal gold tagged antibodies for identifying surface antigens on the surfaces of cultured cells. While the precise details of the protocol one would end up using is very much dependent on the specifics of the samples, we believe that one of the best publications on this topic is the following:
Coller, Barry S., Kutok, J. L., Scudder, L. E., Galanakis, D. K., West, S. M ., Rudomen, G. S., Springer, K. T., "Studies of Activated GPIIb/IIIa Receptors on the Luminal Surface of Adherent Platelets: Paradoxical Loss of Luminal Receptors When Platelets Adhere to High Density Fibrinogen." J. Clin. Invest. Vol. 92, pp.2796-2806, 1993.