SPI-Chem Araldite 6005 Embedding Resin Kit (CofC not available)
Produces blocks that are slightly harder than those made with the Araldite 502. Kit formula is based on method of Glauert, A.M. & Glauert, R.H..
Typical protocols call for an "overnight" cure of 8-16 hours at 80°C.
Kit contains (1450 ml):
Araldite 6005 450 ml
DDSA 450 ml
BDMA 250 ml
DBP (Dibutyl phthalate) 250 ml
Widely popular, world wide, these kits polymerize overnight for easy sectioning and staining the next day. The kits are based on "Lufts formula". We are told that Araldite 502, which is manufactured in the USA, is the same as Araldite 753 which is made in Europe. For many years, the Araldite 502 kits (which we call the "classic" formulation) was widely used world wide for EM procedures. However, for some users, this has been changed slightly, when it was shown that BDMA has a lower viscosity and therefore, more quickly infiltrates tissue samples. Other advantages include a much longer shelf life relative to DMP-30. Also according to Glauert one can add a small amount of DBP in some instances, thereby permitting easier sectioning, since DBP acts as a plasticizer and reduces the hardness of the cured block. On the other hand, there are precautions one can take to lengthen the shelf life of DMP-30, thereby mitigating in some respects that one particular disadvantage.
SPI will continue to supply the widely used SPI-Chem Araldite 502 Kit "original" formulation. We find that this is the formulation requested by most persons. Furthermore, substitution of BDMA for the DMP-30 makes, what would otherwise being a kit not containing a hazardous component, into one that did, resulting in higher shipping charges. But we did want to point out the possibility of using BDMA in place of the DMP-30.
However, for the convenience of those customers who prefer the newer approach using BDMA, we also offer a second version of this popular embedding system. Hence, you can select from the "Original" or the "BDMA enhanced" Araldite 502 kits.
The formulation offered by SPI Supplies for Araldite 502 is identical to that originally published by M. A. Hayat and which was optimized for the following:
- Polymerization by a slow, controlled reaction.
- Optimized polymerization at a temperature for a fixed time.
- No bubble formation during polymerization.
- Formulated for easy sectioning, flexibility and penetrability.
The key is that if one uses 45° C for 24 hours and 60° C for another 24-36 hours, the formulation should work. For heat sensitive samples, one can use only 45°C, but we can not predict how long should be the curing time. We would estimate 72 hours minimum but it could be longer, perhaps as long as 96 hours.
An understated potential safety risk:
We are addressing now the cured block, something that to most people is about as inert of a material as one will find. But the standard practice in many laboratories is to use a small jeweler's (or even a small hack) saw to cut the block down to the right size, sometimes even to shape it. We want to address the dust that is generated and how its exposure can and should be minimized.