SPI Supplies will be closed on Monday, May 30 for Memorial Day. All orders placed during the long weekend will be processed on Tuesday, May 31
Returning customer? Request an account for our new website on the contact form.
There is little documentation of any particular specificity. It is, on the other hand, well known as a metachromatic dye and is used for general staining of semi-thin sections of epoxy and other plastic-embedded materials. The metachromatic dyes are often used to help histochemically characterize glycoaminoglycans (GAGs) and other non-neutral polysaccharides, but toluidine blue O is not usually the first choice for such purposes.
It is also widely used in the Alizarin red/Toluidine blue method for bone and cartilage. This stain also works very well with undecalcified bone as reported in J. of Histotechnology 17, 357-359 (1994).
It can be used as a surface stain, as a stain with plastic removed, or on paraffin sections.
Preparation of the stain:
Great care is needed in the mixing of the 1% toluidine blue solution. There are a number of variations on the protocol, but the following is our suggestion, at least for a starting point: