Conductive Double Sided Adhesive Sheets, Discs, and Tape
Mounting of dry powders for SEM examination
Mounting of dry powders for SEM examination using dry conductive adhesives.
Dr. Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building King's Buildings
Edinburgh EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563
E-mail: cjeffree@srv0.bio.ed.ac.uk
The following tip was passed to me by a user in the Scottish Agricultural
College for spreading a monolayer of starch granules:
1] Dip a polished acrylic plastic rod into the powder and roll it gently
on the surface of a double-sided carbon tab. The use of double sided
conductive tapes or sheets would probably yield essentially the same results.
2] This method has two advantages for the SEM user:
(a) grains can be electrostatically attracted to the surface of the rod
forming a monolayer, and
(b) the rod applies enough pressure to ensure that the particles are stuck
down across a broad base, improving continuity of the metal coating on the
grain with that of the tab surface and SEM mount, minimizing charging.
This approach would probably work for a number of other particulate or
powder systems, but could realistically be expected to work well when the
particles are large(no last minute drying).
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