SPI Supplies

Easy Molds™ for Electron and Light Microscopy

Use instructions

Just follow these five easy steps, that is all there is to it!

Step 1:
Easy adjustment and orientation of the sample in the mold

Prior to polymerization, because of the very thin mold walls, one can easily locate and "see" the specimen and orient it as desired. Indeed the mold walls are almost transparent.

The end result is a "capsule" or "embedment" that can, in fact, also easily accommodate large specimens.



Step 2:
Free unrestricted air flow about the molds for homogeneous and uniform polymerization

Always position the Easy Mold trays for polymerization on a flat and hard surface to be sure of free airflow around the molds for homogeneous and uniform polymerization. When using micro-ovens for the polymerization, the temperature is usually about 60° C but the Easy Molds can be used up to above 85° C. However at the temperature exceeds more and more this temperature, the polyethylene composition starts to undergo an "annealing" process, resulting is a loss of touchness and therefore also, a loss in flexibility of the "push out" feature.


Step 3:
Sealing of the embedments from oxygen

When using any of the acrylate or other resin systems that should not be exposed to oxygen (or which contain volatile components that should be allowed to evaporate away during polymerization) such as "Low Acid" GMA, "Low Acid HPMA", Lowicryl™, LR White™, Unicryl™, then the molds must be "sealed" by placing another Easy Mold on top as indicated to the right, which serves also as a cap. The very best results are obtained when the cavities are almost to the point of slight over filling so that there is not trapped air space and also, the capillary action of the liquid against the bottom of the top tray used for the sealing, really does provide an efficient barrier against oxygen or the escape of volatile components.




Step 4:
Removal of the now polymerized block

To remove the final cured block, simply push up the mold with both thumbs. When things have worked right, the block literally "jumps out". This is made possible by the very thin mold walls at the base of the "pyramid" which is so thin it is virtually transparent. One has no need for razor blades or a capsule press.



Step 5:
Easy retrieval of prepared blocks

There are several options, one being that, since the cavities are all numbered, and since also further identifying information can be written on the trays themselves, the Easy Mold can be used for storing the blocks that were made in it. Since the blocks have already had at least some sections taken off, there is no danger of the bottom of the plastic Easy Mold causing damage to the end of the block if it is reinserted into its same cavity. Alternatively, one can also literally reuse the Easy Mold.




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Tuesday October 07, 2008
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