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Phase contrast applications



Phase contrast microscopy, and a related technique called differential interference contrast (DIC) microscopy, is a low light level condition microscopy, to illustrate topology and also differences in index of refraction. The visualization of the crystalline texture of a crystallizable polymer is one very important example of the use of DIC. It is a contrast enhancing technique based on a phase difference introduced by the addition by a 1/4 wave plate.

Phase contrast is considered a low light level form of light microscopy and therefore depending on your particular samples, you might be using long exposure times and therefore in need of a cooled camera.


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Tuesday February 09, 2010
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