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Dark field microscopy applications


Dark field light microscopy is a commonly used method of light microscopy. Like bright field microscopy, it is used in many scientific applications but in this case, only the target appears illuminated, while the background is black. In this case, one may be concerned about the overall light level, even though the target is usually adequately illuminated. Hence, the sensitivity of the camera might be as critical a consideration. Good color rendition again is very important.

This image has been taken using a Carl Zeiss microscope with Ultra Long Working Distance Objectives (40mm) in dark field. The indent has been created using a Schimatzu micro hardness tester at high temperature (1500 centigrade) so you see the indent burning like amber. The approximate size of the indent (diagonal) is 95 microns.


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Wednesday February 08, 2012
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