Dark field light microscopy is a commonly used method of light microscopy. Like bright field microscopy,
it is used in many scientific applications but in this case, only the target appears illuminated, while
the background is black. In this case, one may be concerned about the overall light level, even though
the target is usually adequately illuminated. Hence, the sensitivity of the camera might be as critical
a consideration. Good color rendition again is very important.
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This image has been taken using a Carl Zeiss microscope with Ultra Long Working Distance
Objectives (40mm) in dark field. The indent has been created using a Schimatzu micro
hardness tester at high temperature (1500 centigrade) so you see the indent burning
like amber. The approximate size of the indent (diagonal) is 95 microns.
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