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KODAK® Electron Microscope Film 4489™

Some suggestions for avoiding problems during developing

As of November 2003, the following was the recommendation from Kodak for obtaining the best results. We cite the date because in the past, this has been a moving target and these instructions are somewhat different from those included with the current manufacture of the film:

Processing:

Recommended developer:
KODAK® Developer D-19 diluted 1 part developer and 2 parts water

Developing time:
4 minutes at 20°C (68°F).
Agitation:
Hand agitate by quickly inserting the film rack into the developer tank, tapping the rack on the tank bottom to dislodge any air bubbles that may cling to the film surface. Every 10 seconds, rapidly remove the film rack completely from the developer tank and tip the film rack 45° to one side of the tank and then tip the film rack 45° to the other side of the tank, allowing the developer to drain into the tank (complete tipping procedure is only a few seconds). Rapidly return the film rack into the tank. Repeat this process throughout the development cycle. Development times longer than 4 minutes will result in a rapid increase in background fog.

Rinse:
For 1.5 minutes in running water at 18 to 21°C (65 to 70°F) with continuous agitation. Do not use conventional stop bath solutions because they may produce a mottled appearance on films used for electron exposures.

Fixer:
Fix as normal done.

Acknowledgement:
We are grateful to Geoff Williams, Microscopy Facility Supervisor, Central Michigan University, Biology Department, Microscopy Facility for posting this information on the Microscopy Listserver on November 20, 2003.

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Wednesday July 09, 2008
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