Material Safety Data Sheet

Date Effective......... February 9, 2009 (most recent revision) Chemical Name/Synonyms Safranin O Basic Red 2; C.I. 50240 3,7-diamino-2, 8-dimethyl-5-phenylpenazinium chloride Emergencies Contacting CHEMTREC 24 Hour Emergency Use Only #'s.... Worldwide phone : 1-(703)-527-3887 Worldwide FAX : 1-(703)-741-6090 Toll-free phone : 1-(800)-424-9300 USA only Product or Trade Name.. Safranin O Synonyms............... 3,7-diamino-2,8-dimethyl-5-phenylphenazinium Chloride; Basic Red 2; C.I. 50240

Hazardous Material Information System USA	Health	2	National Fire Protection Association USA
	Fire Hazard	0
	Reactivity	0
	Personal Protection	0

NFPA Rating: (estimated) Health: 2; Flammability: 0; Reactivity: 0

Component Name CAS # Percent EINECS/ELINCS Safranin O 477-73-6 100 207-518-8 RTECS# SG1623000

Appearance: green-brown solid Flash Point: not available Warning! Causes respiratory tract irritation. Causes eye and skin irritation. The toxicological properties of this material are not fully known. Target Organs: Eyes, skin Potential Health Effects Eye: The toxicological properties of this material are not fully known, but similar cationic dyes have been known to produce permanent corneal or conjunctival injury. Skin: Skin irritant Ingestion: May be irritating to the gastrointestinal tract with symptoms such a nausea, vomiting, and diarrhea Inhalation: Irritating to the respiratory tract. Chronic: The toxicological properties of this material are not fully known.

Eyes: Immediately flush eyes with copious amounts of water for at least 15 minutes, holding the eyelids open. Seek immediate medical attention. Skin: Flush skin with plenty of soap and water for at least 15 minutes. If irritation develops or persists, seek medical attention. Ingestion: Never give anything by mouth to an unconscious person. If victim is conscious and alert, give several cupfuls of milk or water. Seek immediate medical attention. Inhalation: Remove victim to fresh air. If breathing is difficult, supply oxygen. If breathing stops, use artificial respiration.

General Information: Protective Equipment/Clothing: Wear a NIOSH approved positive pressure self-contained breathing apparatus and firefighter turnout gear. There could be the evolution of toxic products of combustion. Firefighting equipment should be fully decontaminated after use. Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or foam.

General Information: Personal precautions: Do not attempt to take action without suitable protective clothing. Do not create dust so that there are air borne particulates that could be inhaled. Environmental precautions: Do not discharge into drains, rivers, or lakes. Release response: Sweep up or vacuum material, place in suitable disposal container, and retain for recycle or disposal. When pickup is complete, wash contaminated area. Do not flush spilled material to the sewer or the environment. Waste materials should be put into a labeled disposal container for disposal according to local regulations. Dispose of in accordance with all federal, state and local regulations.

Handling: Use with adequate ventilation. Wash thoroughly after handling. Do not generate dust. Avoid breathing dust, or contact with skin, eyes, clothing. Wash clothing before reuse. Storage: Store in tightly closed containers in cool, dry area. Keep away from excessive heat and from oxidizing agents.

Engineering Controls: Safety shower and eyewash station required in the work area. Mechanical exhaust sufficient to keep airborne levels low required. Exposure Limits: Chemical Name ACGIH NIOSH OSHA - Final PELs Safranin O n/a n/a n/a OSHA Vacated PELs: n/a Personal Protective Equipment Eyes: Chemical safety glasses or splash goggles. Skin: Appropriate gloves to prevent skin contact. Clothing: Lab coat or other protective clothing to prevent skin contact. Respirators: If irritation or other symptoms are experienced, use A NIOSH/MSHA approved air purifying dust or mist respirator or European Standard EN 149.

Physical State: solid Appearance: green-brown Odor: n/a pH: n/a Vapor Pressure: n/a Vapor Density: n/a Evaporation Rate: negligible Viscosity: n/a Boiling Point: n/a Freezing/Melting Point: decomposes Autoignition Temperature: n/a Flash Point: n/a Decomposition Temperature: n/a Explosion Limits: Lower: n/a Upper: n/a Solubility in water: soluble Specific Gravity/Density: n/a Molecular Formula: C20H19ClN4 Molecular Weight: 350.8

Chemical Stability: Stable Conditions to Avoid: Excessive heat Incompatibility with Other Materials: Strong oxidizing agents Hazardous Decomposition of Products: Hydrogen chloride, carbon monoxide, carbon dioxide, oxides of nitrogen Hazardous Polymerization: Does not occur.

RTECS#: CAS# 477-73-6: SG1623000 LD50/LC50 Information: not available Carcinogenicity: CAS# 477-73-6 is not listed by ACGIH, IARC, NTP, OR California Prop.65. Teratogenicity: not available Reproductive Effects: not available Neurotoxicity: not available Mutagenicity: not available Other Studies: To our knowledge, the toxicological properties of this substance have not been fully investigated.

Exotoxicity: not available Environmental Fate: not available Aquatic: not available Atmospheric: not available Physical: not available

Waste disposal: Arrange for collection by an approved hauler, following federal, state and local regulations. Disposal of packaging: Arrange for disposal following all federal, state, and local regulations. RCRA P-Series: not listed RCRA U-Series: not listed

This product is not classified as hazardous. US DOT Hazard Class: Not regulated. IATA (for international shipments: Not Regulated.

United States: TSCA: CAS# 477-73-6 is listed on the TSCA Inventory. Health & Safety Reporting List CAS# 477-73-6 is not listed on the Health & Safety Reporting List. Chemical Test Rules CAS# 477-73-6 is not under a Chemical Test Rule. Section 12b: CAS# 477-73-6 is not listed under TSCA Section 12b. TSCA Significant New Use Rule: CAS# 477-73-6 is not under a Significant New Use Rule. CERCLA Section 103: CAS# 477-73-6 is not listed. SARA: CAS# 477-73-6 is not listed. Section 302 (RQ) CAS# 477-73-6 does not have an RQ. Section 302 (TPQ) CAS# 477-73-6 does not have a TPQ. SARA Codes Section 313 CAS# 477-73-6 is not listed under SARA Section 313. Clean Air Act: CAS# 477-73-6 does not contain any hazardous air pollutants, any Class 1 Ozone depletors, or any Class 2 Ozone depletors. Clean Water Act: CAS# 477-73-6 is not listed under the Clean Water Act as a Hazardous Substance, as a Priority Pollutant, or as a Toxic Pollutant. OSHA: CAS# 477-73-6 is not listed as highly hazardous by OSHA. State (Individual states in the USA) CAS# 477-73-6 is not listed on any state’s Right to Know List. California Prop. 65: CAS# 477-73-6 is not listed. European/International Regulations: European Labeling in Accordance with EC Directives Hazard Symbols: Xi Harmful, irritant Risk Phrases: R 36 Irritating to eyes. R 38 Irritating to the skin. Safety Phrases: S 24 Avoid contact with skin. S 25 Avoid contact with eyes. WGK (Water Danger/Protection): Not available Canada WHMIS: This product has a WHMIS classification of D2B. DSL/NDSL List: CAS# 477-73-6 is listed on the DSL List. CAS# 477-73-6 is not listed on the DSL List. NOTE: n/a = not available