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Structure Probe, Inc.

P.O. Box 656 West Chester, PA 19381-0656 USA
Phone: 1-(610)-436-5400 Fax: 1-(610)-436-5755
E-mail: spi3spi@2spi.com

WWW: http://www.2spi.com
Manufacturer's CAGE: 1P573

Material Safety Data Sheet

SPI #02589-AB, 02589-MB Safranin O

Safranin O Section 01 Identification

Date Effective......... February 9, 2009
                        (most recent revision)

Chemical Name/Synonyms  Safranin O
                        Basic Red 2; C.I. 50240
                        8-dimethyl-5-phenylpenazinium chloride

Emergencies      Contacting CHEMTREC 24 Hour Emergency 
Use Only #'s.... Worldwide phone : 1-(703)-527-3887
                 Worldwide FAX   : 1-(703)-741-6090
                 Toll-free phone : 1-(800)-424-9300 USA only

Product or Trade Name.. Safranin O
Synonyms............... 3,7-diamino-2,8-dimethyl-5-phenylphenazinium
                        Chloride; Basic Red 2; C.I. 50240

Hazardous Material
Information System
Health 2 National Fire
Protection Association
Fire Hazard 0
Reactivity 0
Personal Protection 0
NFPA Rating: (estimated) Health: 2; Flammability: 0; Reactivity: 0

Section 2 Composition

Component Name      CAS #       Percent     EINECS/ELINCS
Safranin O          477-73-6    100         207-518-8

RTECS# SG1623000 

Section 3: Hazard Identification

Appearance: green-brown solid
Flash Point:  not available 
Warning!  Causes respiratory tract irritation.  Causes eye and skin
irritation.  The toxicological properties of this material are not
fully known.

Target Organs: Eyes, skin

Potential Health Effects

Eye: The toxicological properties of this material are not fully known, but
similar cationic dyes have been known to produce permanent corneal or
conjunctival injury. 

Skin: Skin irritant

Ingestion: May be irritating to the gastrointestinal tract with symptoms such
a nausea, vomiting, and diarrhea

Inhalation: Irritating to the respiratory tract.

Chronic: The toxicological properties of this material are not fully known.

Section 4: First Aid Measures

Eyes: Immediately flush eyes with copious amounts of water for at
least 15 minutes, holding the eyelids open.  Seek immediate medical attention.

Skin: Flush skin with plenty of soap and water for at least 15
minutes.  If irritation develops or persists, seek medical attention.

Ingestion: Never give anything by mouth to an unconscious person.  If
victim is conscious and alert, give several cupfuls of milk or water. Seek
immediate medical attention.

Inhalation: Remove victim to fresh air.  If breathing is difficult,
supply oxygen.  If breathing stops, use artificial respiration.

Section 5: Fire Fighting Measures


General Information:
Protective Equipment/Clothing: Wear a NIOSH approved positive pressure
self-contained breathing apparatus and firefighter turnout gear. There could
be the evolution of toxic products of combustion. Firefighting equipment
should be fully decontaminated after use.

Extinguishing Media:
Use water spray, dry chemical, carbon dioxide, or foam.

Section 6: Accidental Release Measures


General Information:
Personal precautions: Do not attempt to take action without suitable
protective clothing. Do not create dust so that there are air borne
particulates that could be inhaled.

Environmental precautions:
Do not discharge into drains, rivers, or lakes. 

Release response:
Sweep up or vacuum material, place in suitable disposal container, and retain
for recycle or disposal. When pickup is complete, wash contaminated area. Do
not flush spilled material to the sewer or the environment. Waste materials
should be put into a labeled disposal container for disposal according to
local regulations. Dispose of in accordance with all federal, state and local

Section 7: Handling and Storage

Use with adequate ventilation.  Wash thoroughly after handling.  Do not
generate dust.  Avoid breathing dust, or contact with skin, eyes, clothing.
Wash clothing before reuse. 

Store in tightly closed containers in cool, dry area.  Keep away from
excessive heat and from oxidizing agents.

Section 8: Exposure Controls and Personal Protection

Engineering Controls:
Safety shower and eyewash station required in the work area.  Mechanical
exhaust sufficient to keep airborne levels low required.

Exposure Limits:
Chemical Name       ACGIH       NIOSH       OSHA - Final PELs
Safranin O          n/a         n/a         n/a

OSHA Vacated PELs: n/a

Personal Protective Equipment

Eyes: Chemical safety glasses or splash goggles.

Skin: Appropriate gloves to prevent skin contact.

Clothing: Lab coat or other protective clothing to prevent skin contact.

Respirators: If irritation or other symptoms are experienced, use A
NIOSH/MSHA approved air purifying dust or mist respirator or European
Standard EN 149. 

Section 9: Physical and Chemical Properties

Physical State:             solid
Appearance:                 green-brown
Odor:                       n/a
pH:                         n/a
Vapor Pressure:             n/a  
Vapor Density:              n/a
Evaporation Rate:           negligible
Viscosity:                  n/a
Boiling Point:              n/a 
Freezing/Melting Point:     decomposes
Autoignition Temperature:   n/a
Flash Point:                n/a
Decomposition Temperature:  n/a
Explosion Limits:
      Lower:    n/a
      Upper:    n/a
Solubility in water:        soluble 
Specific Gravity/Density:   n/a
Molecular Formula:          C20H19ClN4
Molecular Weight:           350.8  

Section 10: Stability and Reactivity

Chemical Stability: Stable

Conditions to Avoid: Excessive heat

Incompatibility with Other Materials: Strong oxidizing agents

Hazardous Decomposition of Products: Hydrogen chloride, carbon
monoxide, carbon dioxide, oxides of nitrogen

Hazardous Polymerization: Does not occur.

Section 11: Toxicological Information

CAS# 477-73-6: SG1623000

LD50/LC50 Information: not available

CAS# 477-73-6 is not listed by ACGIH, IARC, NTP, OR California Prop.65.  

Teratogenicity: not available

Reproductive Effects: not available

Neurotoxicity: not available 

Mutagenicity: not available

Other Studies: To our knowledge, the toxicological properties of this
substance have not been fully investigated. 

Section 12: Ecological Information

Exotoxicity: not available

Environmental Fate: not available 

Aquatic: not available

Atmospheric: not available

Physical:  not available

Section 13: Disposal Considerations

Waste disposal: Arrange for collection by an approved hauler, following
federal, state and local regulations. 

Disposal of packaging: Arrange for disposal following all federal, state, and
local regulations. 

RCRA P-Series:  not listed
RCRA U-Series:  not listed

Section 14: Transport Information

This product is not classified as hazardous. 

US DOT Hazard Class: Not regulated.

IATA (for international shipments: Not Regulated.

Section 15: Regulatory Information

United States:

TSCA: CAS# 477-73-6 is listed on the TSCA Inventory.

Health & Safety Reporting List
CAS# 477-73-6 is not listed on the Health & Safety Reporting List.

Chemical Test Rules
CAS# 477-73-6 is not under a Chemical Test Rule.

Section 12b:
CAS# 477-73-6 is not listed under TSCA Section 12b.

TSCA Significant New Use Rule:
CAS# 477-73-6 is not under a Significant New Use Rule.

CERCLA Section 103:
CAS# 477-73-6 is not listed.

CAS# 477-73-6 is not listed.

Section 302 (RQ)
CAS# 477-73-6 does not have an RQ.

Section 302 (TPQ)
CAS# 477-73-6 does not have a TPQ.

SARA Codes

Section 313
CAS# 477-73-6 is not listed under SARA Section 313.

Clean Air Act:
CAS# 477-73-6 does not contain any hazardous air pollutants, any Class 1
Ozone depletors, or any Class 2 Ozone depletors.

Clean Water Act:
CAS# 477-73-6 is not listed under the Clean Water Act as a Hazardous
Substance, as a Priority Pollutant, or as a Toxic Pollutant.

CAS# 477-73-6 is not listed as highly hazardous by OSHA.

State (Individual states in the USA)
CAS# 477-73-6 is not listed on any state’s Right to Know List.

California Prop. 65:
CAS# 477-73-6 is not listed.

European/International Regulations:
European Labeling in Accordance with EC Directives

Hazard Symbols:  Xi	Harmful, irritant
Risk Phrases:        
	R 36  Irritating to eyes.
	R 38  Irritating to the skin.  
Safety Phrases:
	S 24  Avoid contact with skin.
	S 25  Avoid contact with eyes.

WGK (Water Danger/Protection):
Not available

This product has a WHMIS classification of D2B. 

CAS# 477-73-6 is listed on the DSL List.
CAS# 477-73-6 is not listed on the DSL List.

NOTE: n/a = not available

Section 16: Other Information

Disclaimer of Liability:
Caution! Do not use SPI Supplies products or materials in applications involving implantation within the body; direct or indirect contact with the blood pathway; contact with bone, tissue, tissue fluid, or blood; or prolonged contact with mucous membranes. Products offered by SPI Supplies are not designed or manufactured for use in implantation in the human body or in contact with internal body fluids or tissues. SPI Supplies will not provide to customers making devices for such applications any notice, certification, or information necessary for such medical device use required by US FDA (Food and Drug Administration) regulation or any other statute. SPI Supplies and Structure Probe, Inc. make no representation, promise, express warranty or implied warranty concerning the suitability of these materials for use in implantation in the human body or in contact with internal body tissues of fluids.


The information and recommendations set forth above are taken from sources believed to be accurate as of the date hereof, however SPI Supplies and Structure Probe, Inc. make no warranty with respect to the accuracy of the information or the suitability of the recommendations, and assume no liability to any user thereof. The information contained in this sheet does not constitute a hazard assessment and should not be used in place of the user's own assessment of work place risks as required by other health and safety legislation.

Be aware of the Structure Probe, Inc. Copyright Policy. Structure Probe, Inc. grants a nonexclusive license to make unlimited copies of this safety sheet for internal use only. Quite obviously, this information would pertain only to this material when purchased from SPI Supplies as product from other sources, with other ingredients and impurity levels could have substantially different properties.

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