SPI Critical Point Drying Apparatus

The specimen holder assembly consists of the following parts:

1. Liquid transfer boat (aluminum alloy) with integral drain valve.
2. Specimen holder baskets which slide into a gauze carrier. The carrier fits in the top of the liquid transfer boat.

Tissue preparation is carried out with the tissue in the baskets to the stage where the tissue is impregnated with the final substitution liquid (i. e. acetone, amyl acetate, etc.). The boat is then filled with the substitution liquid and the basket assembly is transferred rapidly to the boat. The boat can then be loaded into the pressure chamber of the apparatus.

When the specimen access door on the pressure chamber is closed, this actuates the drain valve in the liquid transfer boat. The drain valve aperture is only 0.75mm in diameter and this results in a sufficiently slow draining action for liquid carbon dioxide to enter and cover the specimen before the substitution liquid level has fallen.

Servicing

To strip and clean drain valve in transfer boat:

1. Undo screw in end of transfer boat.
2. Remove valve slider and spring.
3. Clean out drain hole using 1/32"/0.8 mm diameter drill.
4. Refit slider and spring, and retighten screw.

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