SMART GRIDS have a wide variety of potential application and these represent gereral functionized surfaces. Each of these
functionalized grids have been shown to provide even greater control over sample preparation.
Amine For materials sciences, the amine functionality can be used to simplify and enhance sample preparation. For example, it is
possible to simply immerse the grid in a solution of particles that have a negative surface partial charge and they will "stick"
to the surface through electrostatic interactions.
In the life sciences, the amine terminal groups can be used to chemically attach a wide variety of biological molecules including
proteins, antibodies, and nucleic acids through the use of bifunctional cross-linking agents such as imidoesters or N-hydroxysuccinimide-esters
(NHS esters). Similar types of cross linking reagents can be used to covalently attach non-biological media such as nanoparticles
functionalized with biomolecules.
To order SMART Grids, please make your selection below. For assistance in selecting the correct window
size and thickness for your applications, see our configuration page.
HydroxylHydroxyl groups can serve as anchor points for introducing virtually any silane to the surface of the SMART Grids.
In addition, the hydroxyl functionality has a negative partial charge that can capture particles with positive surface
charge, thus allowing for simplified sample preparation. More generally applicable is the fact that the hydroxyl SMART
Grids are extremely hydrophilic (<10 degrees). This extreme hydrophiliicity eliminates effects from drying during sample
prep such as aggregation and drying marks. While several of our other grids are also hydrophilic, these offer the general
capability with a wide range of materials without interference from specific absorption.
Epoxide Epoxides are highly reactive to amine, hydroxyl, thiol and thiocyanates.This reactivity makes epoxide functionalized
SMART Grids a great choice for applications in biology and materials science. Epoxide grids have been used to concentrate
viruses on the grid surface for easy observation and characterization. Note that epoxides hydrolyze quickly and should be
stored in a dessicator prior to use.
Carboxylate Carboxylates can be crosslinked to amines and are excellent hydrogen bonders. Carboxylate functionalized SMART
Grids are a great choice for numerous applications in biology and materials science.
ThiolThiol functionality can be used to form disulfide bonds between the grid surface and biomolecules such as proteins and
antibodies. In addition, thiols show a high affinity for many metal nanoparticles, making them suitable for applications in the nanomaterials field.
Amine The primary alkene found in our alkene functionalized SMART Grids provides greater flexibilty than our
traditional alkane hydrophobic SMART Grids. Use these when the reactivity of a primary alkene is useful for sample preparation.