SPI SEM Quantifoil® Holey Carbon Calibration Specimens
Instructions for mounting on SEM mount
The Quantifoil holey carbon specimens can be mounted on the
SEM mount of your choice, by one of several different methods:
- Double sided conductive adhesive carbon discs
We think this is the best way for mounting the grids.
Use the appropriate size disc and after it is applied to the mount of
choice, carefully place on the exposed carbon adhesive, the grid. It
should be possible to put more than one type of grid onto a single SEM mount
this way, and when the grid needs to be replaced, with the
appropriate tweezers, there should be enough tac remaining to securely
hold the replacement grid.
- Glass cover slip
This is also an excellent alternative. Using
Duro 5-Minute™ Epoxy Glue, affix a glass cover slip to the surface
of your favorite style SEM mount,
and then sputter coat with gold the cover slip and surrounding mount in your
regular sputter coater used for the preparation of your SEM samples. Then,
with a small "dot" of silver paint from the
SPI Silver Kwik-Stik™, in two or three locations on the cover slip,
carefully deposit the grid such that the three "dots" of silver paint will
now be holding it in place.
- Bare SEM mount surface mounting
This method is preferred by those "schooled" in the art of the
"quick and dirty". Just take a tooth pick and sticking it into your
silver paint, put down
a couple of tiny "dots" on the SEM mount surface, attach the Quantifoil
grid to the mount using the silver paint dots, and after a few minutes of
drying, insert into the SEM and begin your examination.
There are other variations of course but we have listed the three most
popular methods of attachment to the SEM mount. Because of the wide
diversity of SEM mounts now available, not to mention the diversity in the
ways people wish to use the grids, we have decided to offer the grids
unmounted, letting the purchaser make these other decisions on the basis of
what makes the most sense for their particular situation.
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Sunday September 07, 2008
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