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Quantifoil® Micromachined Holey Carbon Grids

Cryo-electron tomographic reconstruction


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A reconstruction of Neurospora mitochondria was carried out by Nicastro and colleagues (Max-Planck-Institute for Biochemistry, Martinsreid).

Courtesy of Dr. D. Typke. See for more information: D. Nicastro et. al, Cryo-electron tomography of Neurospora mitochondria, J. Struct. Biol. 129, 48-56, 2000.

An electron micrograph of a zero-degree projection of a tilt series; the edge of the hole in the Quantifoil is seen at the bottom.


A central x-y slice through the reconstructed volume.


A view of the reconstruction, showing that the crista membrane that forms a three-dimensional network of interconnected lamellae is continuous with the inner boundary membrane (both shown in yellow). The outer membrane (magenta) is separated from the inner one by a narrow width of remarkably constant width.

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Sunday March 21, 2010
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