Quantifoil® Micromachined Holey Carbon Grids
Automated image acquisition
The use of Quantifoil® Micromachined Holey Carbon Grids
allows image acquisition to be performed automatically, something not
possible with films of random holes.
The above results were obtained by the Imaging Technology Group of
Dr. Bridget Carragher, University of Illinois, Urbana-Champaign, IL USA.
They have developed a system called Leginon™, for automatically
acquiring images from a transmission electron microscope.
The Procedure:
1. Acquisition of a low magnification image (left image, showing TMV
embedded in vitreous ice suspended over 2 µm holes),
2. Automatic identification of holes containing ice of the suitable
thickness (note the cross in the left image),
3. Acquisition of an intermediate magnification image of an identified hole
(middle),
4. Identification of features of interest within the hole,
5. Focussing at the magnification to be used for the picture and
6. Acquisition of a high magnification image (right, showing microtubules
in this case).
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Sunday March 21, 2010
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