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UNICRYL™ User's Manual

A Universal Resin for Light and Electron Microscopy



Chinese



TABLE OF CONTENTS

SECTIONTITLE
GENERAL DESCRIPTION

1Why Is UNICRYL unique?
2How is UNICRYL to be used?
3How is UNICRYL polymerized?
3.1Polymerization by heat or UV irradiation
3.2Choice of polymerization temperature.
3.3Polymerization at high temperature
3.3.1Typical protocol for high temperature polymerization
3.3.2Typical heat polymerization times for UNICRYL
3.4Polymerization by UV light
3.4.1Polymerization chamber
3.4.2Light arrangements for UV polymerization
(a)Direct or indirect light
(b)UV light intensity
(c)Wavelength of UV light
(d)Polymerization of control samples of resin
(e)Polymerization with stained or colored tissue/td>
3.4.3Typical UV polymerization times for UNICRYL
3.4.4Typical protocol for UV polymerization I
3.4.5Progressive lowering of temperature (PLT)
4What are the cutting properties of UNICRYL?
5What are the labeling and staining characteristics of UNICRYL?
6Trouble shooting
Q1.The resin does not polymerize fully in two days.
Q2.Part of the resin is polymerized and part remains liquid.
Q3.Bubbles appear in the polymerized resin block.
Q4.Sections show ripples.
Q5.Sections dissolve on the water bath.
Q6.The block is brittle.
Q7.Holes appear in sections. Tissue ultrastructure is poor.
Q8.Sections are unstable in the electron beam.
Q9.Labelling is poor.
Q10.Resin hardness varies throughout the tissue.
Q11.Resin was left to polymerize for an excessive time. Will it be damaged?
Q12.Resin appears to evaporate.
7Storage of UNICRYL
8Safety and handling



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Wednesday February 08, 2012
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