SPI-Chem Stains for Light Microscopy - Dry Powder

Safranin O method for cartilage

This method was reported on the Histology Listserver on July 30, 1998, posted by John Difford, FIBMS, Histopathology Department, Royal Free Hospital, London, England:

1. Dewax section and take to water
2. Stain nuclei with a suitable iron haematoxylin
3. Blue in running tapwater
4. Rinse in distilled water
5. Stain with 1% light green diluted 1 in 5 with distilled water, for 3 minutes 6. Rinse in 1% acetic acid
7. Stain with 0.1% Saffranin O, for 4 - 6 minutes
8. Rinse in 1% acetic acid and check under microscope. Any overstaining with Safranin can be modified by re-applying the light green solution briefly, and vice versa.
9. Dehydrate with alcohol, clear and mount

To Ask a Question or Make a Comment

To Place an Order or Request a Quote

Return to: