SPI Supplies

SPI-Chem ™ "Low Acid" GMA Kits Water Soluble

Assures lowest background staining at both the EM and LM levels



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Some representative references
  1. Cole, M. B., Jr., A simple apparatus for ultraviolet polymerization of water-soluble embedding media employed in electron microscopy,
    J. Microsc. (Paris) 7 (3), 441-444 (1968).


  2. Cole, M. B., Jr., and S. M. Sykes, Glycol methacrylate in light microscopy. A routine method for embedding and sectioning animal tissues.
    Stain Technol. 49 (6), 387-400, (1974).


  3. Cole, M. B., Jr., Holding plastic embedded specimens for sectioning in a rotary microtome,
    Experimentia 32 (5) 674-675 (1976).


  4. Cole, M. B., Jr., An "azo" substitute for benzoyl peroxide in polymerizing glycol methacrylate (GMA),
    Micron 10, 135-137 (1979).


  5. Cole, M. B., Jr., and J. Ellinger, Glycol methacrylate in light microscopy. Nucleic acid cytochemistry.
    J. Microsc. (Oxf.) 123, 75-88 (1981).


  6. Cole, M. B., An interlacunar network in fracture callus cartilage. In: The Chemistry and Biology of Mineralized Connective Tissue,
    A Veis, Editor, Elsivier North Holland, New York. Pages 363-365.


  7. Cole, M. B., Jr., Gylcol methacrylate embedding of bone and cartilage for light microscopic staining,
    J. Microsc. (Oxf), 127, 139-148 (1982).


  8. Cole, M. B., Jr., Morphology of the interlacunar network in four sites of hyaline cartilage of neonatal, juvenile and adult rats,
    Clin. Orthoped. Rel. Res. 170, 85-93 (1982).


  9. Cole, M. B., Jr. K. r. Narine and J. Ellinger, Morphological evidence of the shedding or chondrocytes from the articular surface in neonatal rats: Relationship to the interlacunar network,
    Anat. Rec. 206, 439-446 (1983).


  10. Cole, M. B., Alternation of cartilage matrix morphology with histological processing,
    J. Microsc. (Oxf) 133 (2), 129-140 (1984).


  11. Cole, M. B., Jr., Testing methods and results of testing "low acid" glycol methacrylate (GMA) from different sources,
    J. Histochem. Cytochem 32 (5), 555-556 (1984).


  12. Cole, M. B., Jr., Disappearance of the cartilage interlacunar network during histological dehydration.
    Stain Technol. 59 (5), 259-264 (1984).


  13. Cole, M. B., Jr. and K. R. Narine, Staining glycol methacrylate- embedded cartilage with triethyl-carbocyanin DBTC ("Ethyl-Stains-All") with special reference to the interlacunar network.
    Stain Technol. 59 (6), 323- 333 (1984).


  14. Schwarcz, T. H., P. B. Dobrin, M. Mrkvicka, L. Skowron and M. B. Cole, Jr., Early myointimal hyperplasia after balloon catheter embolectomy: Effect of shear forces and multiple withdrawals.
    J. Vasc. Surg. 7, 495-499 (1988).


  15. Cole, M. B. Jr., Methods and results of testing "low acid" glycol methacrylate (GMA) for light microscopic cytochemistry,
    J. Histochem. Cytochem 32: 555-556 (1984).


  16. Cole, M.B., Jr., Testing Methods and Results of "Low Acid" Glycol Methacrylate (GMA) from Different Sources.
    J. Histochem. Cytochem. 32(5):555-556 (1984).


  17. Stirling JW. Unfixed tissue for electron immunocytochemistry: a simple preparation method for colloidal gold localization of sensitive epitopes using ethanediol dehydration.
    Histochem. J. 24:190-206, 1992.


  18. Stirling JW. Immuno-and affinity probes for electron microscopy: a review of labeling and preparation techniques.
    J. Histochem. Cytochem. 38(2):145-157, 1990.


  19. Stirling JW, Coleman M, Brennan J. The use of inert dehydration and glycol methacrylate embedding for immunogold localization of glomerular basement membrane components.
    Lab. Invest. 62(5):655-663, 1990.



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