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SPI-Chem™ Embedding Resin Kits

Should I be using paraffin or plastic?


Chinese


First, this is the kind of question that would be getting asked only of persons studying life science samples. We do not have knowledge of any major applications of paraffin embedment and sectioning for any materials science samples.

With regard to turn around time and the time period needed to obtain good results, both approaches require good infiltration so one would not select one over the other because one was faster.

But plastic embedding has the possibility of making far thinner sections, and therefore one is generally able to have far greater versatility in their approach to the microscopy and this also means ultimately, getting better results.

On the other hand, if one has the need to produce continuous sections of very large samples, then the decision becomes more weighted in the favor of paraffin embedding there is very definitely an upper limit to just how large a sample can be sectioned.

When it comes to hard samples, plastic is always going to be favored out of the need to match to the degree possible the hardness of the embedding resin with the material being embedded. But again for large samples that can be cut only with paraffin, there are various softening methods that can be used so that even originally hard samples can often times be sectioned in paraffin.


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Thursday March 11, 2010
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