The combination of different embedding resins has become popular lately because it blends the best qualities
of each individual' resin into one.
In 1964, Mollenhauer developed an Araldite-Epon (SPI-Pon 812)
mixture for embedding plant tissue, with particular interest in preserving plant cell wall. It has also been
successful with animal tissue. Sectioning with this mixture of resins has been proven to be easier than with each
individual resin and the thermal stability of Araldite and the image contrast of SPI-Pon 812 has made this a very
successful blend.
RECOMMENDED MIXTURE AND PROCEDURE
SPI-PON 812
25 ml
Araldite 6005 DDSA
15 ml
DDSA
55 ml
DMP-30
1.5% or 1.4 ml (added just prior to use)
DEHYDRATION:
Dehydrate tissue in ethanol followed by propylene oxide.
INFILTRATION:
Place tissue in a 2:1 ratio of solvent (propylene oxide or acetone)
to resin mixture (with accelerator), 1 hour at room temperature.
Place tissue in a 1:2 ratio of solvent to with accelerator, 1 hour at room temperature.
Place tissue in 100% resin mixture with accelerator in acids,
2-4 hours at room temperature then in an oven 40-80 unit hard, about 12 hours.
REFERENCES:
Mollehauer, H.H., Stain Technology, 39:111 (1964), Hayat) M.A.,
Principals and Techniques of E.M., V.I, 2nd Edition, University Park Press, Baltimore 1981.
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