QUETOL 651
Instructions for Use:
QUETOL 651 is a water miscible epoxy resin with a low viscosity affording-easy embedding,
infiltration, sectioning and staining. Characterized by low electron scattering,
it can be used for both light and electron microscopy.
Semi-thin sections approximately O.2um thick embedded in QUETOL 651 are suitable
for examination with an ordinary electron microscope operating at l00kV.
This method uses QUETOL 651 and the epoxy resin, nonenyl succinic anhydride (NSA)
and nadic methyl anhydride (NMA) as hardener and DMP-30 as accelerator. QUETOL 651
is an ethylene glycol diglycidyl ether, an epoxy resin of low viscosity. It is a light
colored epoxy resin with a viscosity of 15 cps at 25°C. It is readily miscible with water,
alcohol, acetone, n-butyl glycidyl ether, etc. It acts as a dehydrating agent, and
combines chemically in any cured epoxy resin formulation.
The-following mixture, is recommended for - this method:
| QUETOL 651 | 35 ml |
| NSA | 54 ml |
| NMA | 11 ml |
| DMP-30 | 1.5-2.0 ml |
This mixture has a low viscosity, and is therefore easy to handle and penetrates readily
and completely into the specimen. It sets in about 24 hours at 60°C and yields a light
yellow block which is suitable for sectioning at 20°C. This final hardness of the blocks
can be adjusted by altering the ratio of QUETOL 651 and NSA.
Tissue sample sizes are approximately 2x2x1 mm, since a section of an area of approximately
2x2rnm is suitable for mounting on' a grid 3mm in diameter. The pieces are fixed in buffered
aldehyde solution, post fixed in buffered osmium tetroxide solution, washed in a nonphosphate
buffer, and should then be stained en block with aqueous uranyl acetate. After dehydration in
graded alcohol or QUETOL 651, the following infiltration procedure is appropriate. N-butyl
glycidyl ether (n-BGE) is employed as an auxiliary to infLltration.
All steps are carried out on a shaker at room temperature.
| Embedding: |
| Dehydrating agent/n-BGE) (1:1) | 30 minutes |
| n-BGE | 30 minutes |
| n-BGE/QUETOL 651 mixture (1:1) | 1-2 hr. |
| QUETOL 651 mixture | 2-3 hr. |
Embed in gelatin (or polyethylene) capsules and cure in an at 60°C for about 24 hours.
Kushida, H. and Kushida, T.
J. Electron Microscopy, Vol. 31, No.2, 206-209. 1982
REV. 3/91
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