Pre-mixed and ready to use, the new Monostep Lowicryl Embedding Media saves
time and minimizes chemical contact. Both products are fine for
immunohistochemistry. They are based on
the popular Lowicryl K4M and HM20 kits for low temperature embedding or freeze substitution.
MonoStep products are especially appropriate for immunolabeling resulting in better
preservation of antigenicity and lower background labelling,
but are much more convenient to use than the original Lowicryl kits.
An understated potential safety risk:
We are addressing now the cured block, something that to most people is about as
inert of a material as one will find. But the standard practice in many laboratories
is to use a small jeweler's (or even a small hack) saw to cut the block down to the
right size, sometimes even to shape it.
We want to address the dust that is generated and how its exposure can and should be
minimized.
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