SPI Supplies

SPI-CHEM™ Formaldehyde

Formaldehyde solution, 37% (in water)



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CH2O
Appearance: Clear, colorless liquid
Odor: Pungent odor

Formaldehyde, 37%
CAS # 50-00-0
NIOSH (RTECS) No. LP8925000

Water Wt% by difference, 63%
CAS # 7732-18-5
NIOSH (RTECS) No. ZC0110000

B.P.: 96° C / 205° F
M.P.: -15° C / 5° F
Autoignition temperature: 300° C / 572° F
Vapor Density (Air-1): 1.04
Specific Gravity: 1.08
Solubility in water: Complete
UN2209 Hazard class: 8 Packing Group: III Corrosive

SPI-Chem Formaldehyde has been highly purified for use in the most critical applications in microscopy and histology. Normal technical grade formaldehyde often times contains formic acid which would be detrimental for use in conjunction with electron microscopy.

Additional helpful information:
Pure formaldehyde is a gas which has good solubility in water. A 37% solution of formaldehyde dissolved in water is called formalin. Sometimes this mixtures is called "formaldehyde solution". Normally, various additives are put into the 37% solution of formaldehyde in order to stabilize it, such as methanol. Because of these additives, as well as other impurities, formalin is usually considered unsuitable for electron microscopy studies. Confusion often arises when people refer to percentages of these solutions. For example, a 20% solution of formaldehyde is quite different than a 20% solution of formalin (20% versus 7.4%, respectively).

Formalin is commonly used to describe a mixture of 37% to 38% formaldehyde in water (this reagent grade chemical is also called formaldehyde solution). This solution of dissolved formaldehyde gas generally contains some small amount of methanol in it also. Now for the good part! Traditionally this reagent grade mixture of the 37% or so formaldehyde in water is considered a 100%formalin concentration (the maximum concentration range you can buy commercially). A traditional 10% formalin solution would then contain about 3.7% to 3.8% aldehyde concentration. Many working in electron microscope laboratories prefer to make their formaldehyde solutions from the polymer paraformaldehyde, in part because they believe that there is less methanol in the final product. Yet other workers have reported just fine results using the formalin approach. So a lot might have to do with personal preference than anything else. We hope this bit of comment about these two different approaches might be of interest to those using this material.

MSDS for Braycote 1613
SizeSPI #EachEach, 10+In Stock
Formaldehyde30 ml02614-DA9.558.60 Add to cartYes
450 ml02614-AB12.7311.46 Add to cartYes


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Monday July 28, 2014
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