SPI-Mark™ Cationic Colloidal Gold Conjugates
Use instructions
Cationic Gold for Light and Electron Microscopy
SPI-Mark™ Cationic Gold allows highly sensitive and discrete
microscopical studies of anionic sites in and on cells and tissues. A
simple one step incubation of sections with the diluted gold conjugate at
the correct pH reveals subcellular sites having net negative charge. The
charge distribution may be examined with clear definition at a range of
magnifications in the electron microscope by employing cationic gold of
different sizes or by enhancing gold particles in situ with the SPI-Mark Silver
Enhancing Kit. for Light Microscopy a single size is used and
subsequently silver enhanced.
Most cells of eukaryotic origin have a net negative surface charge from
anionic plasma membrane components. This charge distribution is
thought to be important in the movement of various soluble
macromolecules across cell walls. The role of the surface charge in
cellular behavior through interaction with neighboring cells or with the
intracellular matrix can now be studied microscopically with high
definition by a simple one step procedure. A further advantage of cationic
gold is the possibility of performing labeling studies under various
physiological conditions of pH and ionic strength.
Table of Contents
- Conjugates
- Procedure
- Typical Results
- Controls
- Troubleshooting
- References
- Ancillary Products
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Friday March 12, 2010
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