SPI-Mark™ Cationic Colloidal Gold Conjugates
Controls
Controls
In order to determine specific labeling of anionic sites distinct from
general background, control sections should be first incubated with a
solution of 1mg/ml of unlabelled poly-L-lysine in the same buffer for 1
hour before incubation with the conjugate. Controls should be incubated
under similar conditions and at the same pH as the experimental
sections.
A second valuable control is to use SPI-Mark™ Bovine Serum Albumin Gold
Conjugate at the same dilution and pH in place of the cationic gold
conjugate.
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