SPI-Mark™ Colloidal Gold Reagents
Instructions for Use and Sample Protocols
Washing
The tissue section must again be thoroughly washed with the same buffer to
remove unwanted non-specific gold particles. After washing in buffer the
section must also be thoroughly washed in distilled water to remove all traces
of salt and loose protein before it is allowed to dry.
During the incubations, a greater sensitivity may be achieved by constantly
flushing or agitating the tissue section in the solutions. This may be achieved
on a gentle shaker platform or by immersing the EM section in a small tube
with the incubating solutions and placing it on a roller bed, so bringing fresh
solution continuously to the tissue surface proteins as would be the case in vivo.
If the antibody solutions are tolerant the incubations may also be
performed at 37°C to mimic in vivo conditions further.
If floating sections on the surface of droplets of antibody, it is important to
prevent any antibody solutions from drying on either side of the tissue section.
Drying can produce non-specific aggregations of gold particles across the
section surface.
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Thursday May 17, 2012
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