SPI-Mark™ Colloidal Gold Reagents
Washing
Thorough washing of the tissue following fixation is extremely important. It may
sometimes be necessary to wash for at least as long as the tissue has been
fixed in order to remove excess aldehyde or other fixation residues which may
cause non specific labeling. In some cases quenching the tissue with
ammonium chloride is performed to neutralise aldehyde groups. The wash is
best performed in a buffer having a tonicity similar to the natural tissue.
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Thursday May 17, 2012
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