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Trouble shooting (microscopy)



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Trouble shooting (Microscopy)
Optimisation of incubating conditions will give the maximum signal with the minimum background. Some of the reasons for poor signals and high background in both EM and LM incubations are given here. This list is not exhaustive since so much depends upon the actual protocols used for preparing tissue and incubating sections.

Poor labeling
Excessive Background labeling
Clustering


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Thursday May 17, 2012
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