
SPI-Mark™ Colloidal Gold Reagents
Trouble shooting (microscopy)
Trouble shooting (Microscopy)
Optimisation of incubating conditions will give the maximum signal with the
minimum background. Some of the reasons for poor signals and high
background in both EM and LM incubations are given here. This list is not
exhaustive since so much depends upon the actual protocols used for
preparing tissue and incubating sections.
- Poor labeling
- Excessive Background labeling
- Clustering
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Thursday May 17, 2012
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