SPI-Mark™ Colloidal Gold Reagents
Instructions for Use and Sample Protocols
Secondary Antibody
A high quality secondary antibody is essential to label the primary specifically
and with low background. SPI-Mark™ gold conjugates are affinity purified and are of
the highest quality. The conjugates contain no free antibody, all antibodies
being adsorbed securely to the surface of the gold particles. SPI-Mark™ gold
conjugates need not and should not be centrifuged. Their high titre and purity
means that for incubation they may be highly diluted (typically 1/10 - 1/400) in
the typical incubating buffer shown above (i.e. same as for the primary
antibody), so reducing non-specific background while achieving a high signal
intensity.
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Thursday May 17, 2012
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