SPI-Mark™ Colloidal Gold Reagents
Instructions for Use and Sample Protocols
Primary Antibody
The primary antibody should be of the highest titre and of the highest specific
purity to allow the highest possible dilution. Cross reactivity must be low,
especially with the sample tissue. A low quality primary antibody is the greatest
cause of low specific signal and high background during the labeling
procedure. The buffer composition, and the pH, is important for the
incubations. A normal TBS or PBS buffer is usually chosen with suitable
additions to maintain a low background as described above. Buffer A is
typical. The causes of non-specific background are described below with their
remedies. The dilution of the primary antibody is discussed above. The
specimen must be washed thoroughly with buffer between incubations.
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Thursday May 17, 2012
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