SPI-Mark™ Colloidal Gold Reagents
Etching
If the immunoreactivity is weak or non existent some attempt may be made to
unmask antigens from resin embedded sections for LM or EM in order to make
them more accessible to the antibodies. This masking of antibodies may be
due to cross linking by the aldehyde fixation, chemical interaction with the
resin, covering with other cross linked proteins, osmication, covering of
epitopes with resin, etc. When using etching techniques it is important to avoid
causing further damage to the epitopes while optimally removing the resin or
digesting the surrounding proteins. In general etching is not advised because
of the difficulty of controlling the reaction. For acrylic resins it is almost totally
ineffective anyway.
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Thursday May 17, 2012
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