SPI-Mark™ Colloidal Gold Reagents
Instructions for Use and Sample Protocols
When establishing a new protocol it is necessary to determine the optimum
concentrations of both the primary and the secondary gold labelled antibodies.
This is achieved by first incubating separate sections with various dilutions of
the primary over an appropriate range (e.g. 1/100 - 1/10,000). The various
primary incubations are then followed by second incubations, using a constant
dilution, such as 1/100, of the gold labelled second antibody. The dilution of
the primary antibody, which gives the best signal/background ratio, is thus
determined. The procedure is then repeated, this time using the chosen
primary antibody dilution and varying the gold labelled secondary antibody
dilution over a range of, say, 1/10 - 1/200. In this way the best dilution of both
antibodies will be determined.
To Ask a Question or Make a Comment
To Place an Order or Request a Quote
Return to:
Thursday May 17, 2012
© Copyright 2006 - 2012. By Structure Probe, Inc.
Contacting SPI Supplies and Structure Probe, Inc.
All rights reserved.
All trademarks and trade names are the property of their respective owners.
Privacy Policy
Worldwide Distributors, Representatives, and Agents
