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SPI-Mark™ Colloidal Gold Reagents

The selection of the right colloidal gold conjugate


Chinese

Technical Information and Protocols for use in:

Electron Microscopy
Light Microscopy
Immunoblotting

Contents

1. Introduction
2. Technical Assistance
3. Specimen Preparation
3.1 Fixation
3.2 Washing
3.3 Embedding
3.4 Sectioning
3.5 Etching
3.5.1. Methods of Etching
4. Incubations
4.1 Choice of Gold Conjugate
4.2 Buffer Recipes
4.2.1Incubation Buffer (Buffer A)
4.3 Dilutions
4.4 Incubation Procedure
(I) Blocking
(II)Primary Antibody
(III)Washing
(IV)Secondary antibody
(V) Washing
(VI)Silver Enhancing
(VII)Counterstaining
5. Specific Immunolabelling Protocols
5.1 Post-embedding Labelling Tissues for EM
5.2 Pre-embedding Labelling Tissues for EM
5.3 Pre-embedding Labelling of Cell Surface Antigens for EM
5.4 Double Labelling of EM Sections
5.5 Immunolabelling Following In-situ Hybridisation for EM
5.6 Pre-embedding Labelling of Tissues for LM
5.7 Labelling of Cell Surface Antigens for LM
5.8 Immunolabelling Following In-situ Hybridisation for LM
6. Trouble Shooting (Microscopy)
6.1 Poor Labelling
6.2 Excessive Background Labelling
6.3 Clustering
7. Immunoblotting of Nucleic Acids and Proteins (BL Grade)
7.1 Immunostaining of DNA Gel Transfers (Southern Blots)
7.2 Immunostaining of Protein Gel Transfers (Western Blots)
7.3 Trouble Shooting (Membranes)
8. Controls
9. References


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Thursday February 09, 2012
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