SPI-Chem™ Stains for Electron Microscopy

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Phosphotungstic Acid
Phosphotungstic Acid, N-Hydrate H₃PO₄· 12WO₃ · nH₂O
Appearance: White or yellowish green, slightly efforescent, hygroscopic crystals in form of a powder
Odor: None reported
CAS: #12501-23-4
NIOSH (RTECS) No: TH5650000
MW: 2880.17
Specific Gravity: 3.2
Solubility in Water: 67%
FSC: 6810
Shipping Name: Corrosive solid, acidic, inorganic, n.o.s.
UN#: 3210
Hazard Class: 8
Packing Group: II
Hazard label: Corrosive

PTA is a commonly used negative stain for viruses, polysaccharides, nerves, other biological issue material to be viewed by transmission electron microscopy. There have also been some so-far-as-we-know unpublished reports that PTA can be used for the staining of various nylons, a polymer apparently not readily stained by other electron dense stains. One major advantage of using PTA is that the pH can always be adjusted as desired. Some protocols also call for the use of PTA as a fixative.

For further information, see:
Phosphotungstic Acid Staining of Polysaccharides Containing Structures on Epoxy Embedded Tissues, Bloom, E. E., And Aghajinian, G.K., J. Submicroscopic Cytology 11, 263 (1968).

	SPI #	Each	In Stock
10g	02535-BA	$ 10.98	Yes
100g	02535-AB	55.20	Yes

Storage condition: Room temperature
Hazardous from the standpoint of shipping

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