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Selection of particle size



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Which particle size should be selected?
For the selection of the particle size, it is recommended that this be determined by the magnification to be used in the electron microscope. For the highest labeling intensity, however, the smaller gold particles are preferred. For gold probes 5 nm or less, a combination of gold labeling with silver enhancing will yield larger size particles with high labeling intensity.

For those researchers just starting out, if you are going to try reproducing someone else's work to validate your technique, then obviously, you will want to use the same size as was used in the publications. But if that is not the case, and you are starting out from scratch, we recommend starting with 10 nm gold particles size for observation at a whole range of magnifications.


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Thursday February 09, 2012
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