This is one of those "classic" books that never really seems to go out of
date because it presents an outstanding over view in easy-to-understand and
concise terms.
The volume describes in detail the methods for the fixation, dehydration and
embedding of biological specimens, while techniques for preparing ultrathin
sections and for the staining of the sections to provide contrast in the
transmission electron microscope. In common with other books in this
series , the aim has been to describe the techniques of electron microscopy
in sufficient detail to enable either the isolated worker or the beginning
student to carry them out successfully. Absolutely no specialist knowledge
is assumed.
We have to be honest and point out that the literature survey for this book
ended in 1973 and does not have any sections on some of the more modern
methodologies such as immunogold
staining and microwave fixation
techniques. However, one does have to crawl before they walk and we
at SPI still believe that this book, dated though it might at first appear,
is still great for the TEM beginner. Besides more advanced techniques are
covered in other excellent texts today, but they start out assuming some
fundamental knowledge of life science TEM before hand.
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