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SPI Microscopy Library

Fixation, Dehydration and Embedding of Biological Specimens, Volume 3, Part 1 From the series:
Practical Methods in Electron Microscopy

Author and Editor: Audrey M. Glauert © 1984, 207 pgs., illustrated


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This is one of those "classic" books that never really seems to go out of date because it presents an outstanding over view in easy-to-understand and concise terms.

The volume describes in detail the methods for the fixation, dehydration and embedding of biological specimens, while techniques for preparing ultrathin sections and for the staining of the sections to provide contrast in the transmission electron microscope. In common with other books in this series , the aim has been to describe the techniques of electron microscopy in sufficient detail to enable either the isolated worker or the beginning student to carry them out successfully. Absolutely no specialist knowledge is assumed.

We have to be honest and point out that the literature survey for this book ended in 1973 and does not have any sections on some of the more modern methodologies such as immunogold staining and microwave fixation techniques. However, one does have to crawl before they walk and we at SPI still believe that this book, dated though it might at first appear, is still great for the TEM beginner. Besides more advanced techniques are covered in other excellent texts today, but they start out assuming some fundamental knowledge of life science TEM before hand.

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