This excellent volume incorporates heavy, dense elements into the
appropriate structures under scrutiny in order to provide sufficient
contrast in the transmission electron microscope. Ways in which this
may be achieved are detailed within the pages of this book. Techniques
covered vary from simple, non-specific lead staining to highly specific
methods for enzymes. Topics are arranged by order of increasing specificity
and complexity of use, with an emphasis on the practical details for their
performance in the ordinary electron microscope laboratory.
To help the beginner choose between the sometimes bewildering variety of
published technical procedures, some discussion is included of the
underlying chemical basis of many of the staining techniques. An
acquaintance with histochemistry as used at the light microscope level
would be helpful in understanding more complex staining procedures,
however, the book itself is relatively self-sufficient. Recommended
techniques are given as enumerated procedures which should be easy to
apply with a little care and practice. |
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