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SPI Microscopy Library

Cytochemical Staining Methods for Electron Microscopy
By: P. R. Lewis and D. P. Knight Elsevier © 1992, 321 pgs.,
illustrated From the series: Practical Methods in Electron
Microscopy, Volume 14; Editor : Audrey M. Glauert


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This excellent volume incorporates heavy, dense elements into the appropriate structures under scrutiny in order to provide sufficient contrast in the transmission electron microscope. Ways in which this may be achieved are detailed within the pages of this book. Techniques covered vary from simple, non-specific lead staining to highly specific methods for enzymes. Topics are arranged by order of increasing specificity and complexity of use, with an emphasis on the practical details for their performance in the ordinary electron microscope laboratory.

To help the beginner choose between the sometimes bewildering variety of published technical procedures, some discussion is included of the underlying chemical basis of many of the staining techniques. An acquaintance with histochemistry as used at the light microscope level would be helpful in understanding more complex staining procedures, however, the book itself is relatively self-sufficient. Recommended techniques are given as enumerated procedures which should be easy to apply with a little care and practice.

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