A thorough look at the role of counting and measuring methods in
autoradiography, immunocytochemistry and stereology, along with the
practical and theoretical interrelations among these three areas of
technique. The first section studies stereological techniques, including
specimen preparation; sample selection; obtaining micrographs; measurement
of cellular components, surface area and organelle size; estimating;
determining cell and organelle shape; handling data and quantifying pattern
and organelle arrangement in cells. The second section looks at the
analysis of electron microscope autoradiographs, including the nature of
the image spread; obtaining a suitable tissue sample; first and second
level analytical methods (including circle and hypothetical grain
distribution); the interpretation of kinetic experiments, and absolute
quantitation. |
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