The book begins with three of the world's most eminent electron
immunocytochemists (Edigio, Romano and Horisberger) presenting comprehensive
reviews on the background of ultrastructural immunolocalization, the
techniques and markers available and applications of immunolabelling at the
electron microscopical level. Subsequent chapters then delve into each
well-established technique in clear detail. You'll read John Priestley's
description of the use of immunoenzyme reagents in pre-embedding procedures
and the demonstrated advantages of this approach in the study of central
nervous system arborization; post-embedding immunoenzyme techniques used
by Morel, Pickel and Beaudet; the work of Newman and Jasani with
DNP-labelled ligands in combination with acrylic resins; the use of
colloidal gold, including a look at single and double immunostaining
methods using immunoglobulin-gold; avidin-biotin-marker complexes and
cryoultramicrotomy; freeze-fracture immunocytochemistry; scanning
immunoelectron microscopy and lectin cytochemistry; plus a look at the
problems of "comparative immunocytochemistry"and well as the unique problems
of microbiological identification. |
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